High Resolution HLA typing by sequencing (11 loci)
High Resolution HLA typing by sequencing (11 loci)
TAT: One Week
Using the Third Generation Oxford Nanopore Technology Sequencer
Advantages Over HLA Typing by Luminex
Sequencing
- High resolution technique resolves all patient specific alleles.
- Detects 11 loci (A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1) simultaneously at the same time.
- More specific in providing the relevant information for optimizing donor selection.
- Necessary and extremely accurate for eplet matching on the allelic level.
- High resolution typing of the donor-recipient pairs is relevant for the correct DSA monitoring, denovo DSA and patient management.
Luminex
- Low to intermediate resolution. Resolves all alleles (not patient specific).
- Detects A, B, C, DRB1, DP, and DQ only - separately.
- Less specific than sequencing in providing the relevant information for optimizing donor selection.
- Less accurate in eplet matching on the allelic level.
- Clinician must revise both recipient PRA and donor HLA results to check denovo DSA and manage the patient.